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Transcriptional profiling revealed that a relative large portion of the Arabidopsis and rice NAC TF families exhibited differential expression patterns in response to various biotic and abiotic stresses. Beside the involvement in growth and development, the function of the NAC TFs in biotic and abiotic stress responses has been well documented in both model and crop plants. The NAC TFs contain a highly conserved NAC domain at N-terminal, which determines DNA-binding activity, and a variable domain at C-terminal, which is responsible for transcription activity. NAC proteins are plant-specific TFs and constitute a large family with 151 members in rice. It was also suggested that some of the functionally characterized TF genes may have great potentials in improvement of abiotic stress tolerance in crop plants. Genetic and molecular studies using knockout/knockdown mutants and/or overexpression lines have revealed that many families of TFs such as NAC, AP2/ERF, MYB, WRKY, bZIP, homeodomain, bHLH, NF-Y and CAMTA have members that play roles in abiotic stress response. Such large proportion of differentially expressed genes during a specific abiotic stress response requires a synergistic action of different types of transcription factors (TFs) in both temporal and spatial manners. For example, a total of 5866 genes (2145 up-regulated and 3721 down-regulated), accounting for ~18 % of the annotated genes in rice genome, were differentially expressed during drought stress in rice. Upon perception of environmental stimuli, stress-initiated signaling network often effectively and timely reprograms the expression of a large spectrum of stress-responsive genes. In addition, stress-induced reactive oxygen species (ROS), including hydrogen peroxide (H 2O 2) and superoxide anion, are harmful by-products causing cellular oxidative damage at excess level, whereas ROS are also considered to play signaling roles in regulating abiotic stress response at proper cellular concentration. A number of key genes that are involved in the ABA-dependent and ABA-independent stress pathways have been identified, including DRE-binding protein/C-repeat-binding factor (CBF), ABA-binding factor, MYC and MYB. Abscisic acid (ABA), as a critical stress phytohormone, plays important roles in abiotic stress signaling networks, and the ABA-mediated stress signaling can be divided into ABA-dependent and ABA-independent pathways. Extensive studies have revealed that plants can timely sense external signals and initiate effectively complicated signaling networks to respond to environmental stress by activating various cellular, physiological, biochemical and metabolic processes. Conclusionįunctional analyses through dominant chimeric repressor-mediated suppression of ONAC095 demonstrate that ONAC095 plays opposite roles in drought and cold stress tolerance, acting as a negative regulator of drought response but as a positive regulator of cold response in rice.Įnvironmental constraints such as drought, salt and extreme temperatures often affect adversely plant growth and development, which lead to great loss of productivity worldwide. Furthermore, ONAC095-SRDX plants showed an increased ABA sensitivity, contained an elevated ABA level, and displayed altered expression of ABA biosynthetic and metabolic genes as well as some ABA signaling-related genes. The ONAC095-SRDX plants had decreased water loss rate, increased proline and soluble sugar contents, and up-regulated expression of drought-responsive genes under drought condition, whereas the ONAC095-SRDX plants accumulated excess reactive oxygen species, increased malondialdehyde content and down-regulated expression of cold-responsive genes under cold condition. However, the ONAC095-SRDX plants displayed an improved drought tolerance but exhibited an attenuated cold tolerance. The ONAC095-OE plants showed comparable phenotype to wild type under drought and cold stress conditions. Transgenic rice lines with overexpression of ONAC095 (ONAC095-OE) and dominant chimeric repressor-mediated suppression of ONAC095 (ONAC095-SRDX) were generated. ONAC095 protein had transactivation activity and the C2 domain in C-terminal was found to be critical for transactivation activity. ResultsĮxpression of ONAC095 was up-regulated by drought stress and abscisic acid (ABA) but down-regulated by cold stress. Here, we report the characterization of a rice stress-responsive NAC gene, ONAC095, and the exploration of its function in drought and cold stress tolerance. The NAC (NAM, ATAF and CUC) transcriptional factors constitute a large family with more than 150 members in rice and some of them have been demonstrated to play crucial roles in plant abiotic stress response. Mild steel filler metals offer the advantage of additional crack resistance due to their ductility but the resulting weld. New fabrication is typically performed on material in the annealed or normalized condition. Source: The filler metal recommended for welding 4140 steel is ER80S-D2. Average CVN impact energy on Flash Bainite vs. Post-weld FB tensile specimens failed at/near the interface of weld metal / base metal. Source: Generally it is recommended to weld 4130 in the annealed condition. Note non-uniformity of the ER120 welds without post-weld heat treatment. Source: Pre-heat both the shaft and plate to 600-800 degrees F use stainless 300 series rod cover with insulation blanket or warm sand to slow cool. ![]() Source: If you decide to go that route there is no reason not to have the entire link ht. Source: Ad Improve Your Business ROI - Get A Better Deal On Welding Rod. Its not recommended to weld 4130 or 4140 steel in the quench and tempered or case-hardened conditions without first performing annealing or normalizing in the area to be welded. Is purging going to solve this or is that just delaying the inevitable. Also what is the best type of filler rod to use when welding 4130 to 4130. Low-hydrogen electrodes minimize the amount of diffusible hydrogen in the weld. Abrasion-resistant AR steel plate typically is more resistant to abrasive wear than steel or low-alloy steel plate so its often used in heavy equipment and mining equipment applications. ![]() General guidelines as Ive not done the specific weld you asked about. Welding Question Welding 4130 Steel To Other Metal from Select a filler metal. Do I need to do anything differently when welding 4130 compared to mild steel. While I feel this is a total bullshit way to do it to each his own. ![]() The next page of the manuscript should include the title and a 100-word abstract. Authors’ names and departments should appear only on the title page. The Criminology format for the organization of text, citations and references should be used. Papers may be no longer than no longer than 8,000 words (excluding tables and references). Papers that have been accepted for publication at the time of submission are ineligible.Īpplication Specifications: Papers may be conceptual and/or empirical but must be directly related to criminology. Multiple authored papers are admissible, as long as all authors are students in good standing at the time of submission. Previous prize-winning papers (any prize from any organization and or institution) are ineligible. Dual submissions for the Carte Award and any other ASC award in the same year (including division awards) are disallowed. Students may submit only one paper a year for consideration in this competition. Prior Carte Award first place prize winners are ineligible. These awards are given to recognize outstanding scholarly work of students. The Gene Carte Student Paper Award (established in 1971) is given to recognize outstanding scholarly work of students.Įligibility: Any student currently enrolled on a full-time basis in an academic program at either the undergraduate or graduate level is invited to participate in the American Society of Criminology Gene Carte Student Paper Competition. ![]() Fellowships & Visiting Scholar Positions.Criminology: An Interdisciplinary Journal. The OVA never clarifies whether Monaka is a simple professional assassin or a vigilante seeking justice. Despite its slightly longer length, Kite Liberator has significantly less character development and atmosphere than either the original A-Kite or Mezzo. Apparently the additional time provided by the delay wasn’t enough to salvage the work. In November 2007 Umezu’s anime studio, Arms, announced that the release of Kite Liberator would be delayed in order to give the staff more time to work on the project. It’s sad to say that despite having a longer running time than either A-Kite or Mezzo, all of the action scenes in Kite Liberator combined aren’t as exciting as singular action set pieces from Umezu’s earlier work. Furthermore, the tense, exciting, and death-defying action that characterized A-Kite and Mezzo Forte is noticably absent in Kite Liberator. And the graphic violence in Kite Liberator feels lessened in both frequency and visual impact. While Kite Liberator isn’t entirely sanitized, the graphic sex of Yellow Star, A-Kite, and Mezzo is gone. I don’t know how much, if any American influence compromised the content of Kite Liberator, but plans for an American release certainly do seem to have affected the production. Unlike Umetsu’s previous action anime projects that were produced primarily for Japanese release, Kite Liberator was co-financed by an American distributor and was released practically simultaneously in Japan and America. Regrettably, the long awaited Kite Liberator doesn’t match up to the quality of any of these prior titles, including even the very uneven Mezzo television series. Countless anime fans are also familiar with director Yasuomi Umetsu’s other “girls with guns” anime titles Cool Devices operation 7: Yellow Star, A-Kite, Mezzo Forte, and Mezzo. I have encountered consistent and unanimous confused and disappointed responses to the OVA from viewers that have watched it.įans have anxiously waited ten years since the 1998 release of A-Kite to see a sequel. I’d like to be able to definitively explain what went “wrong” with Kite Liberator, but regrettably I haven’t encountered any statements from either creator/director Yasuomi Umetsu or American co-producer/distributor Media Blasters. ![]() I’d hate to see it just stop right in the middle of everything like this. What’s going on? Is that it? Are there plans for a second episode, or is one likely at any point soon? After so many years, we finally got a sequel to Kite. Well, maybe even ended isn’t the right word it just stopped in the middle of everything and the credits rolled. I was really, really enjoying it and then it just ended. I just finished watching the recent OVA Kite Liberator. ![]() Instead of beads, you could try small candy pieces, flowers and leaves, shiny scraps of foil or colored mylar, or colorful seeds. ![]() You can carefully remove the plastic end and look at other objects through your kaleidoscope.When you turn the tube, what do you notice? Can you see the beads shifting and reflecting to make colorful patterns? Look through the peephole while pointing the bottom of your tube toward the light.Use a hole punch or a sharp pencil to make an eye hole in the center of this circle, then cut it out and tape it to the open end of your tube. Cover the open end of your kaleidoscope with plain cardstock, and trace around your tube to make a circle on the cardstock.Then you can use the rubber band to hold it in place. If you are using plastic wrap, pull it up gently so it is tight. Put your cardboard tube on top of the plastic wrap or lid that contains the beads.If you used a Pringles can lid, or other plastic lid, tape a piece of plastic wrap over the top of it, to keep the beads in place.You will want your plastic wrap to be big enough that you can put your cardboard tube on it and pull it up to cover about 1 inch of the tube on all sides. Make sure that you can still see plenty of light through the plastic. Lay some translucent beads on the plastic wrap, and lightly wrap it over them, so they are held in place, but can still slide around. Take a square of plastic wrap– you can use a sandwich bag (if you don’t have plastic wrap, you can use a small plastic lid, like from a can of Pringles). To make a kaleidoscope, we still have a few more steps to follow.What do you see? Can you see reflections on the inside of the shiny surfaces? ![]()
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Eventually I worked my way up and became a Colony Leader and City Council Member before I left. a party), take screenshots of the virtual world and the avatars of people who had attended, and it would be published in the Daily News along with an article written about the event. One of my favorite jobs was City Photographer - I would show up to events (i.e. I took on quite a few jobs while I was active in the community. The primary focus of the website was simply to hang out and make friends with other users, with all of these unique features serving as a backdrop and generating bustling activity. I feel like I'm barely scratching the surface it was really quite amazing for a creation of the 90s. There was an arcade, bank, flea market, theme park, employment center, a city news organization, etc. There was a Security Chief and Security Deputies who policed the website for inappropriate content and had the ability to suspend or ban users. a lamp or painting), and make money from the people who purchase your product to place in their virtual house. There were other jobs, such as Mall Chief and Mall Deputies, who were in charge of the mall where you could upload your own 3D VRML products (i.e. They made decisions which impacted the whole website, in coordination with the parent company/founder of the website. Sci-fi Colony, Fantasy Colony, Teens Colony, etc.) and the City Council was comprised of the 6 or so Colony Leaders, along with a Mayor and Deputy Mayor. There were something like 6 colonies (i.e. You could start as a Block Deputy, work your way up to Block Leader, Neighborhood Deputy, Neighborhood Leader, Colony Deputy, to Colony Leader. There might be another block in the same neighborhood called Neuromancer. Citizens with an interest in the book Snow Crash would place their home there. A "block" was a collection of homes, a "neighborhood" was a collection of blocks, and a "colony" was a collection of neighborhoods - a sample colony would have been something like "Sci-fi" colony, "Metaverse" neighborhood, and "Snow Crash" block. Block Deputy, in charge of deleting homes owned by inactive users and making more space for new people to move into their block, policing homes with offensive names/content, helping their Block Leader host events for their neighbors, etc.). The part that I was most fascinated with was your ability to start as a new citizen, and seek employment from someone who was hiring for a low-level job (i.e. I've never quite seen it replicated with modern online communities/MMOs and I'm not even sure it would work with today's internet culture. 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